Antigen

Antigen Class

TFs and others

Antigen

NUDT21

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9 cell line

cell line

human embroynic stem cell line H9

chip antibody

10322-1-AP-50ul, Proteintech

genotype

CFIm25 KO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS, and ChIP-seq libraries were prepared using CUT&TagHyperactive In-Situ ChIP Library Prep Kit for Illumina（pG-Tn5）(TD901-01, vazyme) and the indicated antibodies. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 (vazyme, ND607-01) Libraries were sequenced with Novaseq PE150

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

26959519

Reads aligned (%)

38.4

Duplicates removed (%)

35.3

Number of peaks

119 (qval < 1E-05)

hg19

Number of total reads

26959519

Reads aligned (%)

38.2

Duplicates removed (%)

35.5

Number of peaks

88 (qval < 1E-05)