Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NUDT21

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
H9 cell line
cell line
human embroynic stem cell line H9
chip antibody
10322-1-AP-50ul, Proteintech
genotype
CFIm25 KO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS, and ChIP-seq libraries were prepared using CUT&TagHyperactive In-Situ ChIP Library Prep Kit for Illumina(pG-Tn5)(TD901-01, vazyme) and the indicated antibodies. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 (vazyme, ND607-01) Libraries were sequenced with Novaseq PE150

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
26959519
Reads aligned (%)
38.4
Duplicates removed (%)
35.3
Number of peaks
119 (qval < 1E-05)

hg19

Number of total reads
26959519
Reads aligned (%)
38.2
Duplicates removed (%)
35.5
Number of peaks
88 (qval < 1E-05)

Base call quality data from DBCLS SRA